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Yeast Carbon Base without Nitrogen source is used to test the ability of yeasts to assimilate nitrogen by the addition of various nitrogen sources
Cells are grown 1-2 days on pre-sporalation medium before transferring them to sporulation medium
1 gram powdered medium in 1 litre distilled water
5 Final pH 7
5 (4 g/l) Assay ≥99%
Terrific Agar Phosphate Buffered Size:6 x 1kg Yeast Carbon Base without NitrogenFormula g l Tryptone 12 Yeast Extract 24 KH2PO4 9. 4 K2PO4 2. 2 Agar 15 Final pH 7. 0 0. 2 at 25C Suspend 62. 6 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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