Neidhardt Basal Salt Mixture, Glucose 20 g/L Size:658g (20L) Cells are grown 1-2 days

Cells are grown 1-2 days on pre-sporalation medium before transferring them to sporulation medium

Potency  Approximately 640 µ/mg Store dry at 2-8°C

K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium

1  g/L SDS 69

Adams and R

Neidhardt Basal Salt Mixture, Glucose 20 g/L Size:658g (20L) Cells are grown 1-2 daysContaining all micro and macro salt, buffers and glucose. A mixture of Neidhardt basal salt mixture in a concentration of 32. 9 grams l with Neidhardt supplement mixture in a concentration of 3. 1 grams l. Suspend 32. 9 grams of Neidhardt basal salt mixture plus 3. 1 grams of Neidhardt supplement mixture in one litre of distilled water. Sterilisation by means of filtration. After dissolving a slight precipitation might occur. It is not clear what this