Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Tryptone and Yeast are present

Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen

Ammonium Chloride (5 g/l) is replaced by Glutamate (3

15 g SP supplements ( Ade

SIH Amino acid drop-out mixture is based on the Amino acids present in SIH Minimal medium

The extract is an excellent nitrogen source for bacteria

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Tryptone and Yeast are presentSodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room