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2 at 25°C Suspend 20 gram powdered medium in 1 litre distilled water
Store all Drop-out mixtures dry at room temperature
In combination with Drop Out medium supplement mixtures
The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations
Tryptone 10 Yeast extract 5 NaCl 10 Agar 7 Final pH 7
FM drop out mixture, minus Lysine and w/o Methionine, 7100 mg/l Size:250g 2 at 25°C Suspend 20Synthetic Defined Minimal Media This media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed
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