Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g 2 Glucose 20 Tween 80

2 Glucose 20 Tween 80 1 Sodium Acetate   5 Dipotassium Hydrogen Phosphate  2 Manganese sulphate    0

32 KH2PO4 870 Carbon Source   Glucose 10000 Total 20326 Suspend 20

Store all Drop-out mixtures dry at room temperature

2 at 25°C Suspend 27 gram powdered medium in 1 litre distilled water

Suspend 17

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g 2 Glucose 20 Tween 80Manganese sulphate, MnSO4. H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store