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FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies
These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies
Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer
support a luxurious growth of E
NZYDT Agar Size:500g 87 Suspend 19Formula g l Tryptone 10 Yeast extract 5 NaCl 5 MgSO4 anhydrous 0. 49 Thymidine 0. 04 Agar 15 Final pH 7. 0 0. 2 at 25C Suspend 35. 5 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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