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cells cultured in HiDef-ITS can thrive under reduced serum conditions
neural progenitor cells (NPCs)
including CO2 incubation
as repeated supplementation by FGF2 and every day medium change is not required
immortalized cell lines
HiDef® ITS Cell Culture Supplement Size:10 x 10 mL cells cultured in HiDef-ITS canDefined Biosciences HiDef ITS is a defined formulation designed for the routine maintenance and low density attachment of adherent cells under reduced serum conditions. HiDef ITS is a combination of insulin (to stimulate glucose uptake), transferrin (to facilitate iron transport), and sodium selenite (a potent antioxidant and common component supplied by serum), previously shown to reduce the amount of FBS needed in the culture of a wide range of cell
